Sensitive detection of transitional cell carcinoma of the bladder by microsatellite analysis of cells exfoliated in urine.
Identifieur interne : 002205 ( Main/Exploration ); précédent : 002204; suivant : 002206Sensitive detection of transitional cell carcinoma of the bladder by microsatellite analysis of cells exfoliated in urine.
Auteurs : D. Seripa [Italie] ; P. Parrella ; M. Gallucci ; C. Gravina ; S. Papa ; P. Fortunato ; A. Alcini ; G. Flammia ; M. Lazzari ; V M FazioSource :
- International journal of cancer [ 0020-7136 ] ; 2001.
English descriptors
- KwdEn :
- Adult, Aged, Aged, 80 and over, Carcinoma, Transitional Cell (diagnosis), Carcinoma, Transitional Cell (genetics), Carcinoma, Transitional Cell (urine), Female, Humans, Loss of Heterozygosity, Male, Microsatellite Repeats (genetics), Middle Aged, Silver Staining, Trinucleotide Repeat Expansion, Urinary Bladder Neoplasms (diagnosis), Urinary Bladder Neoplasms (genetics), Urinary Bladder Neoplasms (urine).
- MESH :
- diagnosis : Carcinoma, Transitional Cell, Urinary Bladder Neoplasms.
- genetics : Carcinoma, Transitional Cell, Microsatellite Repeats, Urinary Bladder Neoplasms.
- urine : Carcinoma, Transitional Cell, Urinary Bladder Neoplasms.
- Adult, Aged, Aged, 80 and over, Female, Humans, Loss of Heterozygosity, Male, Middle Aged, Silver Staining, Trinucleotide Repeat Expansion.
Abstract
Transitional cell carcinoma (TCC) is the most common bladder tumor. Urine cytology can identify most high-grade tumors but sensitivity is lower if one includes lesions of all grades. Microsatellite marker alterations have been found in many tumor types including bladder cancer and have been used to detect cancer cells in body fluids including urine. The aim of our study is to further evaluate feasibility and sensitivity of microsatellite analysis to detect bladder cancer cells in urine. We studied 55 individuals: 21 with symptoms suggestive of bladder cancer, 23 patients with previous history of TCC and 11 healthy subjects. Genomic DNA was extracted from blood lymphocytes, urine sediment, bladder washings and tumor or normal bladder mucosa. Twenty highly informative microsatellite markers were analyzed for loss of heterozigosity (LOH) and microsatellite instability (MIN) by polymerase chain reaction. Microsatellite analysis of urine identified 33 of 34 (97%) patients with either primary or tumor recurrence, whereas urine cytology identified 27 of 34 (79%) patients (p = 0.0001). Detection of microsatellite abnormalities improved the sensitivity of detecting low-grade and/or stage bladder tumor: from 75-95% for grades G1-G2 and from 75-100% for pTis-pTa tumors. Bladder washings from 25 patients were also analyzed, and in all cases results were identical to those obtained from voided urine. None of the 16 patients without evidence of TCC showed LOH and/or MIN in urine samples or bladder washings. Interestingly, in a patient with persistent bladder mucosa abnormalities, microsatellite alterations were demonstrated 8 months before the histopathologic diagnosis of tumor recurrence. These results further indicate that microsatellite marker analysis is more sensitive than conventional urine cytology in detecting bladder cancer cells in urine and represents a potential clinical tool for monitoring patients with low-grade/stage TCC.
PubMed: 11668518
Affiliations:
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Parrella</name></author><author><name sortKey="Gallucci, M" sort="Gallucci, M" uniqKey="Gallucci M" first="M" last="Gallucci">M. Gallucci</name></author><author><name sortKey="Gravina, C" sort="Gravina, C" uniqKey="Gravina C" first="C" last="Gravina">C. Gravina</name></author><author><name sortKey="Papa, S" sort="Papa, S" uniqKey="Papa S" first="S" last="Papa">S. Papa</name></author><author><name sortKey="Fortunato, P" sort="Fortunato, P" uniqKey="Fortunato P" first="P" last="Fortunato">P. Fortunato</name></author><author><name sortKey="Alcini, A" sort="Alcini, A" uniqKey="Alcini A" first="A" last="Alcini">A. Alcini</name></author><author><name sortKey="Flammia, G" sort="Flammia, G" uniqKey="Flammia G" first="G" last="Flammia">G. Flammia</name></author><author><name sortKey="Lazzari, M" sort="Lazzari, M" uniqKey="Lazzari M" first="M" last="Lazzari">M. Lazzari</name></author><author><name sortKey="Fazio, V M" sort="Fazio, V M" uniqKey="Fazio V" first="V M" last="Fazio">V M Fazio</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2001">2001</date><idno type="RBID">pubmed:11668518</idno><idno type="pmid">11668518</idno><idno type="wicri:Area/PubMed/Corpus">000631</idno><idno type="wicri:Area/PubMed/Curation">000631</idno><idno type="wicri:Area/PubMed/Checkpoint">000589</idno><idno type="wicri:Area/Ncbi/Merge">000089</idno><idno type="wicri:Area/Ncbi/Curation">000089</idno><idno type="wicri:Area/Ncbi/Checkpoint">000089</idno><idno type="wicri:doubleKey">0020-7136:2001:Seripa D:sensitive:detection:of</idno><idno type="wicri:Area/Main/Merge">002284</idno><idno type="wicri:Area/Main/Curation">002205</idno><idno type="wicri:Area/Main/Exploration">002205</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Sensitive detection of transitional cell carcinoma of the bladder by microsatellite analysis of cells exfoliated in urine.</title><author><name sortKey="Seripa, D" sort="Seripa, D" uniqKey="Seripa D" first="D" last="Seripa">D. Seripa</name><affiliation wicri:level="1"><nlm:affiliation>Unità Patologia Molecolare e Terapia Genica, IRCCS H. Casa Sollievo Sofferenza, Opera Padre Pio da Pietrelcina, San Giovanni Rotondo, Italy.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>Unità Patologia Molecolare e Terapia Genica, IRCCS H. Casa Sollievo Sofferenza, Opera Padre Pio da Pietrelcina, San Giovanni Rotondo</wicri:regionArea><wicri:noRegion>San Giovanni Rotondo</wicri:noRegion></affiliation></author><author><name sortKey="Parrella, P" sort="Parrella, P" uniqKey="Parrella P" first="P" last="Parrella">P. Parrella</name></author><author><name sortKey="Gallucci, M" sort="Gallucci, M" uniqKey="Gallucci M" first="M" last="Gallucci">M. Gallucci</name></author><author><name sortKey="Gravina, C" sort="Gravina, C" uniqKey="Gravina C" first="C" last="Gravina">C. Gravina</name></author><author><name sortKey="Papa, S" sort="Papa, S" uniqKey="Papa S" first="S" last="Papa">S. Papa</name></author><author><name sortKey="Fortunato, P" sort="Fortunato, P" uniqKey="Fortunato P" first="P" last="Fortunato">P. Fortunato</name></author><author><name sortKey="Alcini, A" sort="Alcini, A" uniqKey="Alcini A" first="A" last="Alcini">A. Alcini</name></author><author><name sortKey="Flammia, G" sort="Flammia, G" uniqKey="Flammia G" first="G" last="Flammia">G. Flammia</name></author><author><name sortKey="Lazzari, M" sort="Lazzari, M" uniqKey="Lazzari M" first="M" last="Lazzari">M. Lazzari</name></author><author><name sortKey="Fazio, V M" sort="Fazio, V M" uniqKey="Fazio V" first="V M" last="Fazio">V M Fazio</name></author></analytic><series><title level="j">International journal of cancer</title><idno type="ISSN">0020-7136</idno><imprint><date when="2001" type="published">2001</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Adult</term><term>Aged</term><term>Aged, 80 and over</term><term>Carcinoma, Transitional Cell (diagnosis)</term><term>Carcinoma, Transitional Cell (genetics)</term><term>Carcinoma, Transitional Cell (urine)</term><term>Female</term><term>Humans</term><term>Loss of Heterozygosity</term><term>Male</term><term>Microsatellite Repeats (genetics)</term><term>Middle Aged</term><term>Silver Staining</term><term>Trinucleotide Repeat Expansion</term><term>Urinary Bladder Neoplasms (diagnosis)</term><term>Urinary Bladder Neoplasms (genetics)</term><term>Urinary Bladder Neoplasms (urine)</term></keywords><keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>Carcinoma, Transitional Cell</term><term>Urinary Bladder Neoplasms</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Carcinoma, Transitional Cell</term><term>Microsatellite Repeats</term><term>Urinary Bladder Neoplasms</term></keywords><keywords scheme="MESH" qualifier="urine" xml:lang="en"><term>Carcinoma, Transitional Cell</term><term>Urinary Bladder Neoplasms</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Adult</term><term>Aged</term><term>Aged, 80 and over</term><term>Female</term><term>Humans</term><term>Loss of Heterozygosity</term><term>Male</term><term>Middle Aged</term><term>Silver Staining</term><term>Trinucleotide Repeat Expansion</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Transitional cell carcinoma (TCC) is the most common bladder tumor. Urine cytology can identify most high-grade tumors but sensitivity is lower if one includes lesions of all grades. Microsatellite marker alterations have been found in many tumor types including bladder cancer and have been used to detect cancer cells in body fluids including urine. The aim of our study is to further evaluate feasibility and sensitivity of microsatellite analysis to detect bladder cancer cells in urine. We studied 55 individuals: 21 with symptoms suggestive of bladder cancer, 23 patients with previous history of TCC and 11 healthy subjects. Genomic DNA was extracted from blood lymphocytes, urine sediment, bladder washings and tumor or normal bladder mucosa. Twenty highly informative microsatellite markers were analyzed for loss of heterozigosity (LOH) and microsatellite instability (MIN) by polymerase chain reaction. Microsatellite analysis of urine identified 33 of 34 (97%) patients with either primary or tumor recurrence, whereas urine cytology identified 27 of 34 (79%) patients (p = 0.0001). Detection of microsatellite abnormalities improved the sensitivity of detecting low-grade and/or stage bladder tumor: from 75-95% for grades G1-G2 and from 75-100% for pTis-pTa tumors. Bladder washings from 25 patients were also analyzed, and in all cases results were identical to those obtained from voided urine. None of the 16 patients without evidence of TCC showed LOH and/or MIN in urine samples or bladder washings. Interestingly, in a patient with persistent bladder mucosa abnormalities, microsatellite alterations were demonstrated 8 months before the histopathologic diagnosis of tumor recurrence. These results further indicate that microsatellite marker analysis is more sensitive than conventional urine cytology in detecting bladder cancer cells in urine and represents a potential clinical tool for monitoring patients with low-grade/stage TCC.</div></front></TEI><affiliations><list><country><li>Italie</li></country></list><tree><noCountry><name sortKey="Alcini, A" sort="Alcini, A" uniqKey="Alcini A" first="A" last="Alcini">A. Alcini</name><name sortKey="Fazio, V M" sort="Fazio, V M" uniqKey="Fazio V" first="V M" last="Fazio">V M Fazio</name><name sortKey="Flammia, G" sort="Flammia, G" uniqKey="Flammia G" first="G" last="Flammia">G. Flammia</name><name sortKey="Fortunato, P" sort="Fortunato, P" uniqKey="Fortunato P" first="P" last="Fortunato">P. Fortunato</name><name sortKey="Gallucci, M" sort="Gallucci, M" uniqKey="Gallucci M" first="M" last="Gallucci">M. Gallucci</name><name sortKey="Gravina, C" sort="Gravina, C" uniqKey="Gravina C" first="C" last="Gravina">C. Gravina</name><name sortKey="Lazzari, M" sort="Lazzari, M" uniqKey="Lazzari M" first="M" last="Lazzari">M. Lazzari</name><name sortKey="Papa, S" sort="Papa, S" uniqKey="Papa S" first="S" last="Papa">S. Papa</name><name sortKey="Parrella, P" sort="Parrella, P" uniqKey="Parrella P" first="P" last="Parrella">P. Parrella</name></noCountry><country name="Italie"><noRegion><name sortKey="Seripa, D" sort="Seripa, D" uniqKey="Seripa D" first="D" last="Seripa">D. Seripa</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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